Coding

Part:BBa_K357002:Design

Designed by: Anish Kapadia   Group: iGEM10_Toronto   (2010-06-23)

muconolactone Delta-isomerase or muconolactone D-isomerase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for E. coli. A PstI restriction site was present at bp 104, which then disappeared after the sequence optimization.

Source

AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., 

          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,

          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,

          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,

          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,

          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,

          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,

          Duesterhoft,A., Tummler,B. and Fraser,C.

TITLE     Complete genome sequence and comparative analysis of the

          metabolically versatile Pseudomonas putida KT2440

JOURNAL   Environ. Microbiol. 4 (12), 799-808 (2002)


References